It looks like the new Toucan (2.1.0) continues to try to process a file that it was unable to open, leading to the following log entries. Also, the log entries on the failed fseek and stat calls make it look like it was successful, which is misleading (thanks Microsoft and your weird WIN32 status codes.) I think in this case that the first error message is enough.
It would also be nice to do something to highlight error messages. I have less than computer savvy people running sync jobs (long story.) and colors or something would make it easier to understand the log files quickly.
Setting up progress bar
08:27:32 can't open file 'G:\test.tc' (error 32: the process cannot access the file because it is being used by another process.)
08:27:32 can't get seek position on file descriptor -1 (error 0: the operation completed successfully.)
08:27:32 can't find length of file on file descriptor -1 (error 0: the operation completed successfully.)
08:27:32 Failed to copy the file 'G:\test.tc' to 'C:\Backups\Thumbdrive\Toucan.tmp' (error 32: the process cannot access the file because it is being used by another process.)